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nebnext rrna depletion methods  (New England Biolabs)


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    New England Biolabs nebnext rrna depletion methods
    Nebnext Rrna Depletion Methods, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rrna+depletion+method/NEBNext+rRNA+Depletion+Kit+v2/us11976271-356-56-60
    Average 96 stars, based on 194 article reviews
    nebnext rrna depletion methods - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Diabetes Modulates Iodothyronine Deiodinase 2 Expression in the Mouse Retina: A Role for Thyroid Hormone in the Pathogenesis of Diabetic Retinopathy
    Article Snippet: Enrichment of mRNA was done following rRNA depletion method (NEBNext rRNA Depletion Kit (Human/Mouse/Rat), E6310X) and RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (96RXNS) (New England Biolabs, Ipswich, MA, USA; E7760L).

    Article Title: Diabetes Modulates Iodothyronine Deiodinase 2 Expression in the Mouse Retina: A Role for Thyroid Hormone in the Pathogenesis of Diabetic Retinopathy.
    Article Snippet: Enrichment of mRNA was done following rRNA depletion method (NEBNext rRNA Depletion Kit (Human/Mouse/Rat), E6310X) and RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (96RXNS) (New England Biolabs, Ipswich, MA, USA; E7760L).



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    Figure 1: Study design and workflow. Schematic of the sample flow through 2 protocols. The cDNA concentra- tion after stages I and II were represented by the Lib-1 and Lib-2 indices, respectively. While the <t>NEBNext</t> <t>rRNA</t> Depletion method only executed one stage with Illumina NEBNext Ultra II RNA Library Kit, both Lib-1 and Lib-2 serve as the final cDNA concentrations suitable for RNA sequencing.
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    Figure 1: Study design and workflow. Schematic of the sample flow through 2 protocols. The cDNA concentra- tion after stages I and II were represented by the Lib-1 and Lib-2 indices, respectively. While the NEBNext rRNA Depletion method only executed one stage with Illumina NEBNext Ultra II RNA Library Kit, both Lib-1 and Lib-2 serve as the final cDNA concentrations suitable for RNA sequencing.

    Journal: Biomedical Research and Therapy

    Article Title: Optimizing RNA extraction and library preparation from oral squamous cell carcinoma FFPE samples for next-generation RNA sequencing

    doi: 10.15419/bmrat.v10i10.840

    Figure Lengend Snippet: Figure 1: Study design and workflow. Schematic of the sample flow through 2 protocols. The cDNA concentra- tion after stages I and II were represented by the Lib-1 and Lib-2 indices, respectively. While the NEBNext rRNA Depletion method only executed one stage with Illumina NEBNext Ultra II RNA Library Kit, both Lib-1 and Lib-2 serve as the final cDNA concentrations suitable for RNA sequencing.

    Article Snippet: The stage I cDNA concentrations (lib1) were significantly higher with the exome capture-based method than with the NEBNext rRNA depletion method (p < 0.001), possibly because the total RNA was handled less in the exome-capture method (reverse transcription only) than in the rRNA depletionmethod (rRNA depletion and reverse transcription), providing less opportunity for it to degrade.

    Techniques: RNA Sequencing

    Figure 3: The relationship between library cDNA concentration by 2 methods. Lib-1 was the cDNA concen- tration after stage I with the NEBNext RNA library procedure, while Lib-2 was the cDNA concentration after stage II with the xGen hybridization capture procedure.

    Journal: Biomedical Research and Therapy

    Article Title: Optimizing RNA extraction and library preparation from oral squamous cell carcinoma FFPE samples for next-generation RNA sequencing

    doi: 10.15419/bmrat.v10i10.840

    Figure Lengend Snippet: Figure 3: The relationship between library cDNA concentration by 2 methods. Lib-1 was the cDNA concen- tration after stage I with the NEBNext RNA library procedure, while Lib-2 was the cDNA concentration after stage II with the xGen hybridization capture procedure.

    Article Snippet: The stage I cDNA concentrations (lib1) were significantly higher with the exome capture-based method than with the NEBNext rRNA depletion method (p < 0.001), possibly because the total RNA was handled less in the exome-capture method (reverse transcription only) than in the rRNA depletionmethod (rRNA depletion and reverse transcription), providing less opportunity for it to degrade.

    Techniques: Concentration Assay, Hybridization